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anti pstat3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pstat3
    Anti Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pstat3/pm41936612-101-12-14
    Average 86 stars, based on 1 article reviews
    anti pstat3 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes
    Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), anti-SYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), anti-pSTAT3 (CST, 9145S), anti-STAT3 (CST, 9139S), anti-pSTAT5 (CST, 4322S), anti-STAT5 (CST, 94205S), anti-pPI3K pP85/pP55 (CST, 4228 L), anti-PI3K P85 (CST, 4292S), anti-pAKT (CST, 4060 L), anti-AKT (CST, 9272S), anti-pmTOR (CST, 5536S), anti-mTOR (CST, 2983S), anti-pS6 (CST, 4858 L), anti-S6 (CST, 2217S), anti-pFOXO1 (CST, 9461S), anti-FOXO1 (CST, 2880S), anti-CTCF (ABclonal, A19588), anti-SPIB (CST, 14337S), anti-ETS1 (ZenBio, R382074), anti-GABPA (HuaBio, HA500187 ), anti-FLI1 (HuaBio, HA500149 ), anti-PU.1 (ABclonal, A20461), anti-GAPDH (Proteintech, 60004-1-Ig), anti-PIK3C3 (ABclonal, A12295), anti-PTEN (CST, 9188S), anti-pSHIP-1 (CST, 3941S), SHIP-1 (ABclonal, A3571), anti-NOTCH2 (ABclonal, A24877), anti-pHSL (CST, 4126S), anti-HSL (CST, 4107S), anti-ATGL (CST, 2138S), anti-LC3 I/II (ABclonal, A5618), IκBα (ABclonal, A19714), pIκBα (SelleckChem, F2237), pIKKα/β (CST, 2697S), pP38 (SelleckChem, F0159), HSP90 (ABclonal, A5027), pJNK1/2 (ABclonal, AP0473), pERK1/2 (ABclonal, AP0472). ..

    Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes.
    Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), antiSYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), anti-pSTAT3 (CST, 9145S), anti-STAT3 (CST, 9139S), anti-pSTAT5 (CST, 4322S), anti-STAT5 (CST, 94205S), anti-pPI3K pP85/pP55 (CST, 4228 L), anti-PI3K P85 (CST, 4292S), anti-pAKT (CST, 4060 L), anti-AKT (CST, 9272S), anti-pmTOR (CST, 5536S), anti-mTOR (CST, 2983S), anti-pS6 (CST, 4858 L), anti-S6 (CST, 2217S), anti-pFOXO1 (CST, 9461S), anti-FOXO1 (CST, 2880S), anti-CTCF (ABclonal, A19588), anti-SPIB (CST, 14337S), anti-ETS1 (ZenBio, R382074), anti-GABPA (HuaBio, HA500187), anti-FLI1 (HuaBio, HA500149), anti-PU.1 (ABclonal, A20461), anti-GAPDH (Proteintech, 60004-1-Ig), anti-PIK3C3 (ABclonal, A12295), antiPTEN (CST, 9188S), anti-pSHIP-1 (CST, 3941S), SHIP-1 (ABclonal, A3571), anti-NOTCH2 (ABclonal, A24877), anti-pHSL (CST, 4126S), anti-HSL (CST, 4107S), anti-ATGL (CST, 2138S), anti-LC3 I/II (ABclonal, A5618), IκBα (ABclonal, A19714), pIκBα (SelleckChem, F2237), pIKKα/β (CST, 2697S), pP38 (SelleckChem, F0159), HSP90 (ABclonal, A5027), pJNK1/2 (ABclonal, AP0473), pERK1/2 (ABclonal, AP0472). ..

    Article Title: Monoclonal antibody
    Article Snippet: .. Western blot analysis was performed using anti-pSTAT3 and anti-STAT3 antibodies (Cell Signaling technology). .. After incubation with HRP-labeled goat anti-rabbit (Cell Signaling technology) or HRP-labeled goat anti-mouse antibody (Cell Signaling technology), the membranes were detected by enhanced chemiluminescence HRP substrate (Merck).

    Blocking Assay:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. Total RNA for massive sequencing (RNA-seq) and qPCR was isolated using the commercial DNA, RNA, and protein purification kit (Macherey-Nagel, Düren, Germany).

    Article Title: Inflammatory characteristics induced by cellular senescence in the junctional epithelium of aged mice.
    Article Snippet: Objectives: The junctional epithelium (JE) is a specialized barrier that maintains periodontal tissue homeostasis.. Although age-related changes have been reported in other epithelial tissues, the mechanisms underlying ageassociated dysfunction in the JE remain largely unknown.. In this study, the aims were to determine whether there is cellular senescence in the JE during natural aging, and to elucidate the underlying molecular

    Incubation:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. Total RNA for massive sequencing (RNA-seq) and qPCR was isolated using the commercial DNA, RNA, and protein purification kit (Macherey-Nagel, Düren, Germany).

    Article Title: Angiotensin II Receptor-Associated Protein (AGTRAP) Enhances Glioma Cell Survival Through the IL-6/JAK2/STAT3 Pathway and Correlates With an Immunosuppressive Microenvironment.
    Article Snippet: .. Membranes were incubated overnight at 4°C with primary antibodies, including anti- AGTRAP (GeneTex, GTX64875, 1:1000), anti- CDK2 (Beyotime, AF1063, 1:500), anti- Bcl2 (abcam, ab182858, 1:2000), anti- Bax (abcam, ab32503, 1:1000), anti- cleaved- caspase- 3 (abcam, ab2302, 1:1000), anti- IL- 6 (abcam, ab214429, 1:1000), anti- p- JAK2 (CST, 8082, 1:1000), anti- JAK2 (CST, 3230, 1:1000), anti- pSTAT3 (CST, 9145, 1:1000), anti- STAT3 (CST, 9139, 1:1000), and anti- β- actin (abcam, ab8226, 1:2000). .. The following day, membranes were incubated with secondary antibodies and developed using an ECL detection system (Biorad).

    Article Title: Inflammatory characteristics induced by cellular senescence in the junctional epithelium of aged mice.
    Article Snippet: Objectives: The junctional epithelium (JE) is a specialized barrier that maintains periodontal tissue homeostasis.. Although age-related changes have been reported in other epithelial tissues, the mechanisms underlying ageassociated dysfunction in the JE remain largely unknown.. In this study, the aims were to determine whether there is cellular senescence in the JE during natural aging, and to elucidate the underlying molecular

    Expressing:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. Total RNA for massive sequencing (RNA-seq) and qPCR was isolated using the commercial DNA, RNA, and protein purification kit (Macherey-Nagel, Düren, Germany).

    Phospho-proteomics:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. Total RNA for massive sequencing (RNA-seq) and qPCR was isolated using the commercial DNA, RNA, and protein purification kit (Macherey-Nagel, Düren, Germany).

    other:

    Article Title: IL-11-IL6ST-STAT3 signaling defines a convergent inflammatory axis in esophageal cancer subtypes.
    Article Snippet: Images were captured at 0, 24, and 48 hours; the relative wound closure was quantified using ImageJ software.

    Control:

    Article Title: Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell Differentiation
    Article Snippet: .. The following antibodies were used: anti-PIM1 (mouse: Abcam, #ab308006; human: Cell Signaling Technology [CST], #54523S), anti-RORγt (Medical & Biological Laboratories, #M219-3), anti-STAT3 (Abcam, #ab68153), anti-pSTAT3 (CST, #9145), anti-β-ACTIN (Abcam, #ab8226), anti-MICU1 (CST, #12524S), anti-His (CST, #2365S), anti-Flag (CST, #14793S), anti-pan-phospho-serine/threonine (ABclonal, #AP1067), secondary anti-mouse immunoglobulin G (IgG; Boster, #BA1050), secondary anti-rabbit IgG (Boster, #BA1054), and control rabbit IgG for IP (Beyotime, #A7016). .. Human IL-17A enzyme-linked immunosorbent assay (ELISA) kit (Elabscience, #E-EL-H5812), human IL-17F ELISA kit (Elabscience, #E-EL-H4193), human IL-22 ELISA kit (Elabscience, #E-EL-H0106), and human GM-CSF ELISA kit (Elabscience, #E-EL-H0081) were used.



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    Image Search Results


    CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Journal: The FASEB Journal

    Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

    doi: 10.1096/fj.202600333R

    Figure Lengend Snippet: CSPG4 blockade reduces TcdB‐induced pSTAT3 nuclear translocation in enteric glial cells. (A) Representative photomicrographs of pSTAT3 (green) immunostaining, phalloidin (red) as a cytoplasm staining, and DAPI (blue) as a nuclear staining in enteric glial cells exposed to TcdA (50 ng/mL) or TcdB (1 ng/mL) at 18 h incubation in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). Scale bars: 50 μm. (B) Percentage of enteric glial cells ( n = 5, mean ± SEM) with positive nuclear pSTAT3 staining at 18 h of incubation with TcdA and TcdB in the presence of isotype control or anti‐CSPG4 antibodies (0.001 μg/mL). One‐way ANOVA followed by the Tukey test.

    Article Snippet: After washing, cells were incubated overnight at 4°C with primary antibodies against CSPG4 (Abcam, ab275024, 1:400), nectin‐3 (PVRL3, Invitrogen, PA5‐51095, 1:200), LRP1 (Abcam, ab92544, 1:100), NFκB p65 (Santa Cruz Biotechnology, sc‐372, 1:100), or pSTAT3 (R&D Systems, AF4607, 1:100).

    Techniques: Translocation Assay, Immunostaining, Staining, Incubation, Control