anti pstat3 (Cell Signaling Technology Inc)
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Anti Pstat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+pstat3/pm41936612-101-12-14
Average 86 stars, based on 1 article reviews
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Western Blot:Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), anti-SYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes. Article Snippet: Subsequently, cell lysis was carried out with RIPA lysis buffer (Beyotime, P0013B). .. The cell lysates were examined via SDS-PAGE, and immunoblotting was performed with the following antibodies: anti-pSYK (Cell Signaling Technology, CST, 2710S), antiSYK (CST, 13198S), anti-pCD19 (CST, 3571S), anti-CD19 (CST, 90176S), anti-β-actin (Proteintech, 66009-1-Ig), anti-pNFκB pP65 (CST, 3033S), anti-NFκB P65 (CST, 4764S), anti-pSTAT1 (CST, 9167S), anti-STAT1 (CST, 14994S), Article Title: Monoclonal antibody Article Snippet: .. Western blot analysis was performed using Blocking Assay:Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH. Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, Article Title: Inflammatory characteristics induced by cellular senescence in the junctional epithelium of aged mice. Article Snippet: Objectives: The junctional epithelium (JE) is a specialized barrier that maintains periodontal tissue homeostasis.. Although age-related changes have been reported in other epithelial tissues, the mechanisms underlying ageassociated dysfunction in the JE remain largely unknown.. In this study, the aims were to determine whether there is cellular senescence in the JE during natural aging, and to elucidate the underlying molecular Incubation:Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH. Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, Article Title: Angiotensin II Receptor-Associated Protein (AGTRAP) Enhances Glioma Cell Survival Through the IL-6/JAK2/STAT3 Pathway and Correlates With an Immunosuppressive Microenvironment. Article Snippet: .. Membranes were incubated overnight at 4°C with primary antibodies, including anti- AGTRAP (GeneTex, GTX64875, 1:1000), anti- CDK2 (Beyotime, AF1063, 1:500), anti- Bcl2 (abcam, ab182858, 1:2000), anti- Bax (abcam, ab32503, 1:1000), anti- cleaved- caspase- 3 (abcam, ab2302, 1:1000), anti- IL- 6 (abcam, ab214429, 1:1000), anti- p- JAK2 (CST, 8082, 1:1000), anti- JAK2 (CST, 3230, 1:1000), Article Title: Inflammatory characteristics induced by cellular senescence in the junctional epithelium of aged mice. Article Snippet: Objectives: The junctional epithelium (JE) is a specialized barrier that maintains periodontal tissue homeostasis.. Although age-related changes have been reported in other epithelial tissues, the mechanisms underlying ageassociated dysfunction in the JE remain largely unknown.. In this study, the aims were to determine whether there is cellular senescence in the JE during natural aging, and to elucidate the underlying molecular Expressing:Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH. Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, Phospho-proteomics:Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH. Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, other:Article Title: IL-11-IL6ST-STAT3 signaling defines a convergent inflammatory axis in esophageal cancer subtypes. Article Snippet: Images were captured at 0, 24, and 48 hours; the relative wound closure was quantified using ImageJ software. Control:Article Title: Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell Differentiation Article Snippet: .. The following antibodies were used: anti-PIM1 (mouse: Abcam, #ab308006; human: Cell Signaling Technology [CST], #54523S), anti-RORγt (Medical & Biological Laboratories, #M219-3), anti-STAT3 (Abcam, #ab68153), |
